Translational_Unit

Part:BBa_K3837013:Design

Designed by: Sungwook Lee   Group: iGEM21_KUAS_Korea   (2021-10-01)


Vio operon induced by IPTG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1831
    Illegal EcoRI site found at 2915
    Illegal XbaI site found at 1567
    Illegal XbaI site found at 4875
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 4699
    Illegal PstI site found at 2456
    Illegal PstI site found at 3496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1831
    Illegal EcoRI site found at 2915
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 4699
    Illegal PstI site found at 2456
    Illegal PstI site found at 3496
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1831
    Illegal EcoRI site found at 2915
    Illegal BamHI site found at 180
    Illegal XhoI site found at 1331
    Illegal XhoI site found at 4639
    Illegal XhoI site found at 5530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1831
    Illegal EcoRI site found at 2915
    Illegal XbaI site found at 1567
    Illegal XbaI site found at 4875
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 4699
    Illegal PstI site found at 2456
    Illegal PstI site found at 3496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1831
    Illegal EcoRI site found at 2915
    Illegal XbaI site found at 1567
    Illegal XbaI site found at 4875
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 4699
    Illegal PstI site found at 2456
    Illegal PstI site found at 3496
    Illegal NgoMIV site found at 1250
    Illegal NgoMIV site found at 1755
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gibson assembly will be used for this part


Source

genes are from the vio plasmid

References